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KMID : 0377519830080010087
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Chung-Ang Journal of Medicine
1983 Volume.8 No. 1 p.87 ~ p.95
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Distribution of Superoxide Dismutase in Varios Organs of Mouse
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Lee Jeung-Yun
Kwon Nyoun-Soo
Lee Hi-Sung
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Abstract
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The subcelluar localization of superoxide dismutase(EC 1.15.1.1) was investigated in various organs of mouse. Mouse organs were fractionated into mitochondrial and cytosolic fractions by differential centrifugation. The activity of superoxide dismutase was measured by the method of McCord and Fridovich. The results were summarized as follows ; 1. Mouse organs contain two types of superoxide dismutase, one of which is localized in the mitochondria and the other was found in the cytosol. 2. Superoxide dismutase activity was found in all mouse organs investigated. Maximal activity of enzyme was observed in testicle, kidney and adrenal gland. 3. Most of superoxide dismutase activity was present in the cytosolic fraction(88~95%) , the rest being associated with mitochondria. 4. The cytosolic superoxide dismutase was found that contains copper and zinc and similar to the other cupro-zinc superoxide dismutase which was have been isolated from diverse eukaryotes. In contrast, the mitochondrial superoxide dismutase contains to be a manganoprotein. 5. The activity of cytosolic superoxide dismutase of testicle was most prominent in testicle than those of other tissues. And however, that of mitochondrial superoxide dismutase was highest in the adrenal gland. 6. The specific activity of superoxide dismutase in mitochondria was found about 5 fold greater than that of superoxide dismutase in cytosol. 7. 5mM cyanide inhibit the activity of cytosolic superoxide dismutase to 50~75%, but mitochondrial superoxide dismutase was inhibited to 9%.
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